[1]李晓琴,张自峰,王雨生,等. 脂多糖诱导炎症对大鼠氧诱导视网膜病变的影响[J].眼科新进展,2015,35(4):301-304.[doi:10.13389/j.cnki.rao.2015.0081]
　LI Xiao-Qin,ZHANG Zi-Feng,WANG Yu-Sheng,et al. Effects of LPS-induced inflammation on oxygen-induced retinopathy in rats[J].Recent Advances in Ophthalmology,2015,35(4):301-304.[doi:10.13389/j.cnki.rao.2015.0081]
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 脂多糖诱导炎症对大鼠氧诱导视网膜病变的影响
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
35卷
期数:
2015年4期
页码:
301-304
栏目:
实验研究
出版日期:
2015-04-05

文章信息/Info

Title:
 Effects of LPS-induced inflammation on oxygen-induced retinopathy in rats
作者:
 李晓琴; 张自峰; 王雨生; 高翔; 杨湘敏; 徐文芹
 710032　陕西省西安市，第四军医大学西京医院眼科、全军眼科研究所
Author(s):
 LI Xiao-Qin;  ZHANG Zi-Feng;  WANG Yu-Sheng;  GAO Xiang;  YANG Xiang-Min ; XU Wen-Qin
 Department of Ophthalmology , Eye Institute of Chinese PLA , Xijing Hospital, the Fourth Military Medical University, Xi ’ an 710032 , Shaanxi Province . China
关键词:
 ; 炎症; 氧诱导视网膜病变; 视网膜新生血管; 早产儿视网膜病变
Keywords:
 ; inflammation;  oxygen-induced retinopathy ;  retinal neovascular ;  retinopathy of prematurity
DOI:
10.13389/j.cnki.rao.2015.0081
文献标志码:
A
摘要:
 目的　观察不同剂量脂多糖（ｌｉｐｏｐｏｌｙｓａｃｃｈａｒｉｄｅ，ＬＰＳ）诱导炎症对大鼠氧诱导视网膜病变（ｏｘｙｇｅｎ-ｉｎｄｕｃｅｄｒｅｔｉｎｏｐａｔｈｙ，ＯＩＲ）的影响，以探讨炎症在早产儿视网膜病变（ｒｅｔｉ-ｎｏｐａｔｈｙｏｆｐｒｅｍａｔｕｒｉｔｙ，ＲＯＰ）中的作用。方法　将新生ＳＤ大鼠随机分组，实验组依据ＬＰＳ注射剂量的不同分为ＬＰＳ-５０、ＬＰＳ-１００和ＬＰＳ-５００组，并分别设立正常对照组（Ｎ组）和氧诱导对照组（ＯＩＲ组）。Ｎ组幼鼠喂养于空气中，实验组和ＯＩＲ组幼鼠饲养于氧气体积分数为８０％／２１％（２４ｈ交替一次）的氧箱中至出生后１４ｄ（Ｐ１４），随后移至空气中继续饲养。实验组幼鼠行ＯＩＲ处理过程中，于Ｐ７行ＬＰＳ腹腔注射，ＬＰＳ-５０、ＬＰＳ-１００和ＬＰＳ-５００组注射剂量分别为５０μｇ?ｋｇ－１、１００μｇ?ｋｇ－１和５００μｇ?ｋｇ－１。检测各组幼鼠在Ｐ７（注射前）、Ｐ８、Ｐ１１和Ｐ１４等不同时间点的体质量变化；并分别于Ｐ１４和Ｐ１８制作全视网膜铺片，通过ＧＳ-ｉｓｏｌｅｃｔｉｎＢ４染色观察各组视网膜血管化及病理性新生血管情况。结果　在各检测时间点，各实验组和ＯＩＲ组幼鼠的体质量均低于Ｎ组（Ｐ＜０．０５）。Ｐ７时，各实验组和ＯＩＲ组体质量无差别（Ｐ＞０．０５）；至Ｐ８、Ｐ１１和Ｐ１４时，ＬＰＳ-５００组幼鼠体质量明显低于ＯＩＲ组和其他实验组（Ｐ＜０．０５）。Ｐ１４时，Ｎ组幼鼠的浅层视网膜血管已覆盖整个视网膜；而ＯＩＲ组和实验组幼鼠的视网膜均存在无血管区，且ＬＰＳ-５００组无血管区面积最大（２７．３２％ ±３．５８％，Ｐ＜０．０１）。Ｐ１８时，Ｎ组视网膜血管网层次结构清晰；ＯＩＲ组和实验组幼鼠的视网膜均出现病理性新生血管，且ＬＰＳ-５００组新生血管累及视网膜的范围最广（累及钟点数为６．８３±１.７２，Ｐ＜０．０１）。结论　ＬＰＳ诱导炎症可加重大鼠ＯＩＲ，且在一定范围内呈剂量依赖性，提示炎症可能参与ＲＯＰ病理过程。
Abstract:
 Objective To investigate the role of inflammation in retinopathy of prematurity ( ROP) by assessing the effects of different doses of lipopolysaccharide ( LPS) on oxygen-induced retinopathy ( OIR) in rats. Methods Newbom Sprague Dawley rats were randomly divided into treatment group, OIR and normal control groups, and treatment group was subdivided into LPS-50, LPS-100 and LPS-500 subgroups according to doses of LPS injected. Rats of treatment and OIR groups were exposed to alternating 24-hour cycles of hyperoxia ( 80% 02 ) and normoxia ( 21% 02) for 14 days , while rats of normal control group were maintained in room air. Inflammation in pups of treatment groups was induced by intraperitoneal injection of LPS at postnatal day 7 (P7) , and the doses of LPS for LPS-50, LPS-IOO, and LPS-500 groups were 50 Vg . kg ’l , 100 Vg . kg -l and 500 yg . kg -l , respectively. Body mass of all pups were monitored at P7 ( pre-injection) . P8 . P11 and P14. Wholemounted and GS-isolectin B4 stairuung retinas were analyzed for severity of avascular area and retinal neovascularization at P14 and P18. Results Compared with normal control group , the body mass of pups from treatment and OIR groups were decreased at all time points tested (P < 0. 05 ) . At P8 , P11 and P14 , the body mass of rats in LPS-500 group were significantly lower than those of OIR and other LPS-treated rats (P < 0. 05 ) . At P14 . the retinal vessels obliterated in the rats of LPS-treated and OIR groups, with the largest retinal avascular area in LPS-500 group ( 27. 32% + 3. 58% . P < 0. 01 ). At P18 . LPS-treated rats and OIR rats displayed overgrowth of abnormal retinal vessels , and the severity of pathologic neovascularization was increased evidently after LPS (500 Vg ’ kg ") injected (6. 83 + 1. 72 , P < 0. 01 ) . Conclrision LPS-induced inflammation can aggravate OIR in rats in a dose-dependent manner , which indicates that inflammation might participate in the pathology process of ROP.

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备注/Memo

备注/Memo:
 国家自然科学基金资助（编号：８１２７１０１４、８１４７０６５５）；德国洪堡基金会（ＡｌｅｘａｎｄｅｒｖｏｎＨｕｍ-ｂｏｌｄｔＦｏｕｎｄａｔｉｏｎ）仪器设备捐赠基金资助（ｔｏＹＳＷａｎｇ，Ｖ-８１５１／０２０８５）
更新日期/Last Update: 2015-03-31