[1]周琼,刘章,陈娟. 细胞型朊蛋白在不同月龄小鼠视网膜中表达的实验研究[J].眼科新进展,2015,35(2):108-110.[doi:10.13389/j.cnki.rao.2015.0029]
　ZHOU Qiong,LIU Zhang,CHEN Juan. Expression of cellular prion protein in retina of mice with different months old[J].Recent Advances in Ophthalmology,2015,35(2):108-110.[doi:10.13389/j.cnki.rao.2015.0029]
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 细胞型朊蛋白在不同月龄小鼠视网膜中表达的实验研究
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
35卷
期数:
2015年2期
页码:
108-110
栏目:
实验研究
出版日期:
2015-02-05

文章信息/Info

Title:
 Expression of cellular prion protein in retina of mice with different months old
作者:
 周琼; 刘章; 陈娟
 330006　江西省南昌市，南昌大学第一附属医院眼科
Author(s):
 ZHOU Qiong ;  LIU Zhang ; CHEN Juan
 Department of Ophthalmology , the First Affiliated Hospital of Nanchang University. Nanchang 330006 , Jiangxi Province , China
关键词:
 ; 细胞型朊蛋白; 视网膜; β-淀粉样蛋白
Keywords:
 ; cellular prion protein ; retina;  amyloid β-protein
DOI:
10.13389/j.cnki.rao.2015.0029
文献标志码:
A
摘要:
 目的　观察细胞型朊蛋白（ｃｅｌｌｕｌａｒｐｒｉｏｎｐｒｏｔｅｉｎ，ＰｒＰｃ）在２周龄、９个月龄、１８个月龄正常ＩＣＲ小鼠视网膜中的表达情况。方法　选取２周龄、９个月龄、１８个月龄ＩＣＲ小鼠各２０只，取出视网膜分别使用ＲＴ-ＰＣＲ检测视网膜组织中ＰｒＰｃ基因的表达情况，Ｗｅｓｔｅｒｎｂｌｏｔ检测小鼠视网膜组织中ＰｒＰｃ蛋白的表达情况。分析小鼠视网膜中ＰｒＰｃ的表达水平与月龄的关系及１８个月龄小鼠视网膜与脑组织中ＰｒＰｃ的表达差异。结果　２周龄、９个月龄、１８个月龄小鼠视网膜组织中ＰｒＰｃ基因表达的相对光密度值分别为０．１７５±０．０１０、０．４２０±０．０４４、０．６９２±０．０３０，两两比较，差异均有统计学意义（均为Ｐ＜０．０５）；１８个月龄小鼠脑组织中ＰｒＰｃ基因表达的相对光密度值为１．３６３±０．２５５，与１８个月龄小鼠视网膜组织中ＰｒＰｃ基因表达的相对光密度值比较，差异有统计学意义（Ｐ＜０．０５）。２周龄、９个月龄、１８个月龄小鼠视网膜组织中ＰｒＰｃ蛋白表达的相对光密度值分别为０．３１９±０.０２８、０．６９８±０．０６６、０．９９６±０．０５８，两两比较，差异均有统计学意义（均为Ｐ＜０．０５）；１８个月龄小鼠脑组织中ＰｒＰｃ蛋白表达的相对光密度值为２．００８±０．１９２，与１８个月龄小鼠视网膜组织中ＰｒＰｃ蛋白表达的相对光密度值比较，差异有统计学意义（Ｐ＜０.０５）。结论　正常小鼠视网膜中存在ＰｒＰｃ的表达，且表达水平随着小鼠月龄的增加而逐渐增强，但弱于脑组织中的ＰｒＰｃ表达强度。
Abstract:
 Objective To observe the expression of cellular prion protein ( PrP’ ) in retina of ICR mice with 2 weeks old.9 months old and 18 months old. Methods The ICR mice with 2 weeks old.9 months old and 18 months old were chosen,20 cases in each month age ,the retina were extracted.and the expression of Prpe gene and protein were detected by RT-PCR and Western blot. The relationship between expression levels of PrP’ in the mouse retina and month age was analyzed, and the difference of Prpe expression rn retina and brain tissue of ICR mice with 18 months old was also analyzed. Results The relative optical density value of Prpe gene expression in retina of mice with 2 weeks old,9 months old and 18 months old were 0. 175 + 0. 010 .0. 420 + 0. 044 and 0. 692 + 0. 030 , respectively, there were statistical differences between each two month age ( all P < 0. 05 ) . The relative optical density value of Prpe gene expression in brain tissue of ICR mice with 18 months old was l. 363 +0. 255 , there was statistical difference compared with Prpe gene expression in retina (P < 0. 05 ) . The relative optical density value of Prpe protein expression in retina of mice with 2 weeks old.9 months old and 18 months old were 0. 319 +0. 028 ,0. 698 +0. 066 and 0. 996 + 0. 058 , respectively , there were statistical differences between each two month age ( all P < 0. 05 ) . The relative optical density value of PrPe protein expression in brain tissue of ICR mice with 18 months old was 2. 008 + 0. 192 . there was statistical difference compared with Prpe protein expression in retina (P < 0. 05 ) . Conclusion The expression of PrPe exists in normal mouse retina.and the expression level increase with increasing month age of mice , but the expression in retina is weaker than that in brain tissue.

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备注/Memo

备注/Memo:
 国家自然科学基金项目（编号：８１４６００９２）
更新日期/Last Update: 2015-02-03