[1]付书华,詹昶,仇晶晶,等.HSP60在早期糖尿病大鼠晶状体上皮细胞的表达[J].眼科新进展,2015,35(1):022-24.[doi:10.13389/j.cnki.rao.2015.0006]
　FU Shu-Hua,ZHAN Chang,QIU Jing-Jing,et al.Expression of HSP60 in lens epithelial cells in early diabetic rats[J].Recent Advances in Ophthalmology,2015,35(1):022-24.[doi:10.13389/j.cnki.rao.2015.0006]
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HSP60在早期糖尿病大鼠晶状体上皮细胞的表达
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
35卷
期数:
2015年1期
页码:
022-24
栏目:
实验研究
出版日期:
2015-01-05

文章信息/Info

Title:
Expression of HSP60 in lens epithelial cells in early diabetic rats
作者:
付书华; 詹昶; 仇晶晶; 张倩; 游志鹏; 刘菲
３３０００６　江西省南昌市，南昌大学第二附属医院眼科
Author(s):
FU Shu-Hua;  ZHAN Chang;  QIU Jing-Jing;  ZHANG Qian;  YOU Zhi-Peng;  LIU Fei
Department of Ophthalmology，the Second Affiliated Hospital of Nanchang University，Nanchang 330006，Jiangxi Province，China
关键词:
HSP60; 糖尿病; 晶状体上皮细胞
Keywords:
HSP60 ;  diabetes ; lens epithelial cells
DOI:
10.13389/j.cnki.rao.2015.0006
文献标志码:
A
摘要:
目的　观察热休克蛋白（ｈｏｔｓｈｏｃｋｐｒｏｔｅｉｎ，ＨＳＰ）在早期糖尿病大鼠晶状体上皮细胞的表达及变化，探讨ＨＳＰ６０在糖尿病性白内障病变中的作用。方法　鼠龄为６～８周的ＳＤ大鼠随机分成糖尿病组及对照组，每组１８只，糖尿病组在禁食１２ｈ后一次性腹腔注射１０ｇ?Ｌ－１链脲佐菌素诱发糖尿病模型，成功诱导糖尿病模型后４周、８周、１２周分别应用免疫组织化学法及Ｗｅｓｔｅｒｎｂｌｏｔ分析晶状体上皮细胞上ＨＳＰ６０的表达变化。结果　对照组大鼠晶状体上皮细胞上未发现有ＨＳＰ６０表达，糖尿病组大鼠在造模后４周、８周、１２周均可见晶状体上皮细胞上有ＨＳＰ６０表达，两组间有显著差异（均为Ｐ＜０．０５）。糖尿病组大鼠造模后４周和８周间表达量无明显差异（Ｐ＞０．０５）；而１２周时其表达量开始减少，和４周及８周相比较，差异均有统计学意义（均为Ｐ＜０．０５）。结论　早期糖尿病大鼠晶状体上皮细胞有大量ＨＳＰ６０表达，而且随着时间的延长，表达量呈下降趋势。
Abstract:
Objective To explore the role of hot shock protein 60 ( HSP60) in diabetic cataract through observing the expression and change of HSP60 in lens epithelial cells in early diabetic rats. Methods The 6 - 8 weeks old SD rats were divided into normal control group and diabetes group, 18 cases in each group. 10 g . L-l STZ were intraperitoneal injected to induce diabetic model in experimental group , At 4 weeks . 8 weeks, 12 weeks after STZ-induced.the expression and change of HSP60 in lens epithelial cells were analyzed by immunohistochemistry and Western blot in normal control rats and diabetic rats. Results There was no HSP60 expression in lens epithelial cells in normal rats. At 4 weeks .8 weeks ,12 weeks after STZ-injected , there were HSP60 expression in lens epithelial cells in diabetic rats , the difference between two groups was statistical significant ( all P < 0. 05 ) . The amount of expression began to decrease at 12 weeks after STZ-injected , there were significant differences compared with 4 weeks and 8 weeks ( all P < 0. 05 ) .but there was no significant difference between 4 weeks and 8 weeks (P> 0. 05). Conclusion There are a great number of HSP60 expression in lens epithelial cells in the early diabetic rats .but with the extension of time , the expression quantity decline.

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备注/Memo

备注/Memo:
江西省教育厅课题资助（Ｎｏ：ＧＪＪ１１３３１）
更新日期/Last Update: 2015-01-04