[1]宋鸿雁,仇保丰,刘春,等.应用16SrDNA克隆文库法分析B6-Co小鼠角膜炎细菌组成[J].眼科新进展,2014,34(12):1124-1127.[doi:10.13389/j.cnki.rao.2014.0312]
　SONG Hong-Yan,QIU Bao-Feng,LIU Chun,et al.Bacterial composition of B6-Co mice with bacteria keratitis analyzed with 16S rDNA clone library[J].Recent Advances in Ophthalmology,2014,34(12):1124-1127.[doi:10.13389/j.cnki.rao.2014.0312]
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应用16SrDNA克隆文库法分析B6-Co小鼠角膜炎细菌组成
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
34卷
期数:
2014年12期
页码:
1124-1127
栏目:
实验研究
出版日期:
2014-12-05

文章信息/Info

Title:
Bacterial composition of B6-Co mice with bacteria keratitis analyzed with 16S rDNA clone library
作者:
宋鸿雁; 仇保丰; 刘春; 朱顺星; 缪进; 王生存; 吴刘成; 王旭; 邵义祥
226001　江苏省南通市，南通大学实验动物中心南通大学比较医学研究所
Author(s):
SONG Hong-Yan ;  QIU Bao-Feng ;  LIU Chun ; ZHU Shun-Xing ;  MIAO Jin ;  WANG Sheng-Cun ; WU Liu-Cheng ;  WANG Xu ;  SHAO Yi-Xiang
Laboratory Animal Center of Nantong University , Nantong University Institute of Comparative Medicine, Nantong 226001 , Jiangsu Province , China
关键词:
细菌性角膜炎; 16SrDNA克隆文库; 细菌组成; B6-Co小鼠
Keywords:
bacteria keratitis ; 16S rDNA clone library ; bacteria composition
DOI:
10.13389/j.cnki.rao.2014.0312
文献标志码:
A
摘要:
目的　鉴定Ｂ６-Ｃｏ突变系小鼠角膜的病原菌组成，并与人类细菌性角膜炎病原菌组成进行比较分析。方法　选取１０ｄ龄Ｂ６小鼠和Ｂ６-Ｃｏ小鼠各６只为实验动物，提取小鼠角膜组织刮取物细菌基因组ＤＮＡ，通过ＰＣＲ扩增构建１６ＳｒＤＮＡ克隆文库，采用随机测序法分析Ｂ６-Ｃｏ小鼠角膜细菌组成，Ｂ６小鼠作为对照。结果　Ｂ６-Ｃｏ角膜混浊小鼠中共鉴定出２０种细菌，隶属于１５个属，包括葡萄球菌属、假单胞菌属、链球菌属、微球菌属、棒状杆菌属等。葡萄球菌属和假单胞菌属为优势群，葡萄球菌属丰度为１９．７％ ～５３．１％，假单胞菌属的丰度为１７．４％ ～３２．８％。其中葡萄球菌属主要是表皮葡萄球菌，丰度为１３. ８％ ～２０. ９％；缓慢葡萄球菌，丰度为１４．０％ ～３０．９％；金黄色葡萄球菌，丰度为７．４％ ～２０．３％。此外，棒状杆菌和微球菌也比较常见。１０ｄ龄Ｂ６小鼠未检测出细菌。结论　Ｂ６-Ｃｏ突变系小鼠角膜细菌组成与人类角膜炎病原菌相似，是研究人类角膜炎诊断方法、发病机理及临床用药的很好的动物模型。
Abstract:
Objective To investigate the bacterial composition in the eyes of B6-Co mice with bacteria keratitis . and compare with that in humans with bacteria keratitis. Methods Six lO-day-old C57BL/6 ( B6 ) and C57BL/6-Corneal opacity ( B6-Co) mice,respectively , were chosen as experimental animals. Total bacterial genomic DNA was extracted from corneal scrapings of B6 and B6-Co mice. 16S rDNA clone libraries from PCR products were constructed. The bacterial composition from corneal scrapings of B6-Co mice with bacteria keratitis was determined by sequence analysis. B6 mice were used as controls. Results The microbiota analysis detected 20 species in the eyes of B6-Co mice with bacterial keratitis ,including 15 genera such as Staphylococcus, Pseudomonas ,Streptococcus, Corynebacterium and Micrococcus. The donunant genera were Staphylococcus and Pseudomonas and accounted for 19. 7% - 53. 1% and 17. 4% - 32. 8% , respectively. Staplzylococcus included Staphylococcus epidermidis, Staphylococcus lentus and Staphylococcus aureus. which accounted for 13. 8% - 20. g% .14. 0% - 30. g% and 7. 4% - 20. 3% , respectively. In addition, Corynebacterium and Micrococcus were also frequently detected in the eyes of B6-Co mice. No bacteria was detected in lO-day-old B6 mice controls. Conclusion The bacterial composition in the eyes of B6-Co nuce is similar to that in humans with bacteria keratitis ,which suggests that B6-Co mouse is a favorable model to study diagnostic methods , pathogenesis and clinical medication of bacteria keratitis in humans.

相似文献/References:

[1]胡卫萍,徐永根,倪利洋,等.细菌性角膜炎951例病原学及药物敏感性分析[J].眼科新进展,2019,39(10):976.[doi:10.13389/j.cnki.rao.2019.0223]
　HU Wei-Ping,XU Yong-Gen,NI Li-Yang,et al.Pathogenicity and drug sensitivity analysis of 951 patients of bacterial keratitis[J].Recent Advances in Ophthalmology,2019,39(12):976.[doi:10.13389/j.cnki.rao.2019.0223]

备注/Memo

备注/Memo:
江苏省自然科学基金项目（编号：ＢＫ２０１０２７９）；江苏省高校自然科学基金项目（编号： １３ＫＪＢ１８００１９）；南通大学自然科学类科研基金项目（编号：１２Ｒ０８２）
更新日期/Last Update: 2014-12-04