[1]周小煦,宋文静,苏畅,等.异氟醚单凭吸入麻醉法用于糖尿病大鼠玻璃体注射的实验研究[J].眼科新进展,2014,34(8):710-712.[doi:10.13389/j.cnki.rao.2014.0194]
　ZHOU Xiao-Xu,SONG Wen-Jing,SU Chang,et al.Anesthesia effect of isoflurane on intravitreal injection of diabetes rats[J].Recent Advances in Ophthalmology,2014,34(8):710-712.[doi:10.13389/j.cnki.rao.2014.0194]
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异氟醚单凭吸入麻醉法用于糖尿病大鼠玻璃体注射的实验研究
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
34卷
期数:
2014年8期
页码:
710-712
栏目:
实验研究
出版日期:
2014-08-05

文章信息/Info

Title:
Anesthesia effect of isoflurane on intravitreal injection of diabetes rats
作者:
周小煦; 宋文静; 苏畅; 吴建国
天津市，天津医科大学基础医学院超微病理室
Author(s):
ZHOU Xiao-Xu; SONG Wen-Jing; SU Chang; WU Jian-Guo
关键词:
异氟醚; 吸入麻醉; 糖尿病大鼠; 玻璃体注射
Keywords:
isoflurane ;  inhalation anesthesia ;  diabetes rats ;  intravitreal injection
DOI:
10.13389/j.cnki.rao.2014.0194
文献标志码:
A
摘要:
目的　探究异氟醚单凭吸入麻醉法用于糖尿病大鼠玻璃体注射的可行性。方法２０只同龄ＳＤ雄性糖尿病大鼠随机平均分为Ａ、Ｂ组，Ａ组腹腔注射水合氯醛（４ｍｇ? ｋｇ－１）麻醉，Ｂ组采用体积分数１．５％异氟醚吸入法麻醉。麻醉起效后，于显微镜下行大鼠玻璃体注射间充质干细胞。比较２组动物的麻醉起效时间、肛温、呼吸频率、麻醉时间、死亡率、玻璃体注射操作成功率。结果　麻醉后，Ａ、Ｂ两组动物麻醉起效时间分别为（３．７５±０．６３）ｍｉｎ、（４．７８±０．４７）ｍｉｎ，肛温分别为（３４．７０±０．４３）℃、（３５．１８±０．２２）℃，呼吸频率分别为（５８．５６±４．６４）次?ｍｉｎ－１、（５６．８０±２．６１）次?ｍｉｎ－１，差异均无统计学意义（均为Ｐ＞０．０５）。Ａ、Ｂ组麻醉时间分别为（２７．３３±３．８７）ｍｉｎ、（４．８４±０．７５）ｍｉｎ，差异有统计学意义（Ｐ＜０．０５）。除Ａ组１只麻醉死亡、Ｂ组有１例晶状体扎伤外，其他大鼠均成功进行了玻璃体注射，２组的死亡率和操作成功率差异均无统计学意义（均为Ｐ＞００５）。结论　异氟醚吸入法操作简便，成功率高，可作为糖尿病大鼠短时间操作（如玻璃体注射等）的首选麻醉。
Abstract:
Objective To observe the feasibility of isoflurane anesthesia on intravitreal injection of diabetes rats. Methods After inducing diabetes models, twenty male SD rats were randomly and evenly divided into two groups : the anesthesia of rats in group A with intraperitoneal injection of chloralhydrate (4 mg . kg ’l ) ; B inhalation anesthesia with isoflurane ( 1. 5% ) . Intravitreal injections of mesenchymal stem cell were operated in all animals after then. The index of rats’ onset anesthesia time, anal temperature . breath rate . anesthesia time . mortality and experimental effect were observed in the two groups of diabetes rats. Results The onset anesthesia time of group A and B were ( 3. 75 + 0. 63 ) minutes and ( 4. 78 + 0. 47 ) minutes , anal temperature were ( 34. 70 +0. 43 ) oC and ( 35. 18 +0. 22 ) oC ,breath rate were ( 58. 56 +4. 64 ) times per minute and ( 56. 80 + 2. 61 ) times per minute , respectively , there was no significant difference between two groups ( all P > 0. 05 ). The anesthesia time of group A and B were ( 27. 33 + 3. 87 ) minutes and (4. 84 + 0. 75 ) minutes, respectively , there was statistical difference ( P < 0. 05 ) . Furthermore , the mortality and experimental effect also showed no significant difference between two groups(P > 0. 05 ) . Conclusion The isoflurane anesthesia is simple and safe for diabetes rats accepting intravitreal injection.

备注/Memo

备注/Memo:
天津医科大学校级青年基金（编号：２０１３ＫＹＱ０９）
更新日期/Last Update: 2014-07-30