[1]张万虎,刘轩,李晶明,等.OCTA与FFA在小鼠脉络膜新生血管模型中应用的比较研究[J].眼科新进展,2021,41(7):628-632.[doi:10.13389/j.cnki.rao.2021.0129]
　ZHANG Wanhu,LIU Xuan,LI Jingming,et al.Comparison of consistency between optical coherence tomography angiography and fundus fluorescein angiography in a mouse model of laser-induced choroidal neovascularization model[J].Recent Advances in Ophthalmology,2021,41(7):628-632.[doi:10.13389/j.cnki.rao.2021.0129]
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OCTA与FFA在小鼠脉络膜新生血管模型中应用的比较研究/HTML
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《眼科新进展》[ISSN:1003-5141/CN:41-1105/R]

卷:
41卷
期数:
2021年7期
页码:
628-632
栏目:
实验研究
出版日期:
2021-07-05

文章信息/Info

Title:
Comparison of consistency between optical coherence tomography angiography and fundus fluorescein angiography in a mouse model of laser-induced choroidal neovascularization model
作者:
张万虎; 刘轩; 李晶明; 谢安明; 康前雁; 陈丽; 齐赟; 胡霄霏
710000　陕西省西安市，西安交通大学第一附属医院东院（张万虎）； 710061　陕西省西安市，西安交通大学第一附属医院眼科（刘轩，李晶明，谢安明，康前雁，陈丽，齐赟，胡霄霏）
Author(s):
ZHANG Wanhu1; LIU Xuan2; LI Jingming2; XIE Anming2; KANG Qianyan2; CHEN Li2; QI Yun2; HU Xiaofei2
1.East District,the First Affiliated Hospital of Xi’an Jiaotong University ，Xi’an 710000,Shaanxi Province, China
2.Department of Ophthalmology,the First Affiliated Hospital of Xi’an Jiaotong University ,Xi’an 710061，Shaanxi Province, China
关键词:
荧光素眼底血管造影; 光学相干断层扫描血管成像; 脉络膜新生血管; 小鼠模型
Keywords:
fluorescein fundus angiography;  optical coherence tomography angiography;  choroidal neovascularization; mouse model
分类号:
R773.4
DOI:
10.13389/j.cnki.rao.2021.0129
文献标志码:
A
摘要:
目的　以脉络膜铺片为金标准，探讨小鼠脉络膜新生血管（CNV）模型中荧光素眼底血管造影（FFA）与光学相干断层扫描血管成像（OCTA）描述小鼠CNV形态准确性的差异。方法　应用激光诱导制作小鼠CNV模型，在激光造模后第7、14天分别利用FFA、OCTA进行小鼠眼底扫描，并与小鼠脉络膜铺片结果进行对比，寻找与脉络膜铺片一致性最高的检查技术。结果　CNV模型小鼠眼底经测量的FFA、眼底激光成像、OCTA与脉络膜铺片中激光斑的面积，通过ICC一致性分析发现：以脉络膜铺片作为金标准，与它一致性最显著的为FFA，眼底激光成像次之，缺乏一致性的为OCTA。以此得出结论：对于小鼠CNV模型眼底CNV的形态，FFA检查与脉络膜铺片的一致性较好；OCTA与脉络膜铺片的一致性弱于FFA。结论　OCTA、FFA以及多重眼底照相都可以描述小鼠CNV的形态，但FFA仍是描述CNV形态较为准确的工具。
Abstract:
Objective　To compare the consistency of fundus fluorescein angiography (FFA) and optical coherent tomography angiography (OCTA) for detecting lesion area with whole mount in vitro staining with isolectin-IB4 as gold standard in a mouse model of laser-induced choroidal neovascularization (CNV).Methods　Adult mice were subjected to laser to induce a model of CNV. Fundus was scanned and CNV areas were measured by FFA and OCTA on day 7 and 14. CNV areas were measured in all different imaging modalities after laser and were compared to choroidal-RPE whole mounts to find out a better consistency test technology.Results　After the calculation of the choroidal neovascularization area measurements in FFA imagine, multicolor imagine, OCTA and flat-mount, ICC correlation analysis showed there was a high correlation between FFA and flat-mount staining, while there was a poor consistency between OCTA and flat-mount staining. Therefore, it was concluded that for the CNV area in laser-induced CNV,FFA showed a better consistency with flat-mount staining than that between OCTA and flat-mount staining.Conclusion　It is found that FFA and OCTA can be used to evaluate the CNV area in laser-induced mice. However, FFA has a better consistency with flat-mount staining than that between OCTA and flat-mount staining.

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备注/Memo

备注/Memo:
国家自然科学基金项目（编号：81800824）；陕西省自然科学基础研究计划项目（编号：2020JM-400）
更新日期/Last Update: 2021-07-05